School of Veterinary Medicine

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About School of Veterinary Medicine

Who works at the School of Veterinary Medicine

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Dr. ADEL EMHIMMID MOHAMMED SAEIDAH
School of Veterinary Medicine - Azzaytuna University
people
Mr. Ahmed Asaid Abdelrahman Elkady
School of Veterinary Medicine - Azzaytuna University

Publications

Some of publications in School of Veterinary Medicine

(Extraction palm kernel oil from palm kernel using super-critical carbon dioxide(SC-CO2

Extraction of palm kernel oil(PKO) from dehulled ground palm kernel using supercritical carbon dioxide (SC-CO2) was studied at conditions of 313.2 and 353.2 K and at pressures from 20.7 to 48.3 MPa. The yield of PKO increased with pressure from 34.5 to 48.3 MPa at353.2K and attained a value of 49 g oil/100 g palm kernel at 48.3 MPa and 353.2 K
ADEL EMHIMMID MOHAMMED SAEIDAH, Moftah Massaud Bennama Bennama, (3-2017)

El-Muzghi, A. A. M., Mirkov, I., Djokic, J., Aleksandrov, A.P., Miljkovic, D., Glamoclija, J., Kataranovski, D. & Kataranovski, M. (2013). Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats. Immunobiology, 218(12), 1514-1523.

Abstract Rat models of pulmonary aspergillosis are used widely in diagnostic studies and in exploring antifungal therapeutic modalities, but there is lack of data concerning antifungal immunity in rats. In this study, cytokine response to pulmonary infection to Aspergillus fumigatus in non-immunosuppressed rats is explored. Temporal display (from the start of infection up to its eradication) of proinflammatory cytokines (IFN-γ and IL-17) as well as Th2/anti-inflammatory ones (IL-4 and IL-10) was explored by measuring their presence in the environment in which elimination of infection occur (lung homogenates), by production of these mediators by lung cells (recovered by enzyme digestion or by bronchoalveolar lavage) as well as by cells of draining lymph nodes (as sites of generation of cytokine-producing cells). Reduction of infection (1 × 107 conidia) was associated with an increase of IFN-γ and IL-17 content in lung homogenates, but with unchanged IL-4 and IL-10 content. Lung cells produced proinflammatory cytokines with differential dynamics (IFN-γ earlier than IL-17). Differential pattern of Th2/anti-inflammatory cytokine production by lung cells was observed (unchanged IL-4 and increased IL-10), with the levels of the latter higher than proinflammatory cytokines. Upregulation of IFN-γ, IL-17 and IL-10 production and gene expression, but downregulation of IL-4, by draining lymph node cells (dLN cells) accounted essentially for the observed ex vivo cytokine response in lungs. Similar pattern of cytokine production by dLN cells following restimulation with A. fumigatus conidia confirmed the specificity of cytokine response to the fungus. Draining lymph node CD4+ cells seems to be the main source of proinflammatory cytokines, significant contributors to IL-10 production and the target for down regulation of IL-4. The knowledge of immune-based mechanisms of defense against A. fumigatus in rats might be helpful in the future use of rat models of pulmonary aspergillosis particularly those that develop immune-based therapeutic interventions as an adjunct treatment of fungal diseases.
Amal Atia Mhfuod El- Muzghi, (12-2013)

Popov Aleksandrov, A., Mirkov Ivana .,El-Muyghi, A. A. M., Glamodija Jasmin M Miljkovic Danijela Pokic J Ninkov Marina Kataranovski Dragan S & Kataranovski Milena V (2012) Differntial mechanisms of resistance to pulmonary Aspergillus fumigatus Infecti

Nitric oxide (NO) produced by inducible nitricoxide synthase (iNOS) within the central nervous system (CNS)mainly exerts adverse effects in neuroinflammation. Experimentalautoimmune encephalomyelitis (EAE), an animal model of multiplesclerosis, is a neuroinflammatory disorder. CXCL12 is a chemokine,whose antiinflammatory role in EAE has been described recently. Itsmajor producers in the CNS are astrocytes and endothelial cells ofmicro blood vessels (MBV). The aim of this work was to investigate invitro and in vivo effects of NO on CXCL12 gene expression in ratastrocytes and MBV.Materials and methods: Spinal cords (SC) were isolated from rats thatdeveloped EAE at different stages of the disease, homogenized andassessed for iNOS and CXCL12 gene expression by ‘real time’ RT-PCR.In vitro studies were performed on MBV isolated from SC of healthy,non-immunized adult rats, astrocytes derived from neonatal rats andC6 cells (rat astroglioma). After stimulation with supernatantscollected from splenocyte cultures stimulated with concanavalin Afor 48 h or with combination of cytokines (IL-1b + TNF + IFN-c + IL-17) they were exposed to NO donor, sodium-nitroprusside(SNP). Also, peritoneal cells isolated from healthy rats were co-cultivated with astrocytes. Nitrite accumulation in supernatants, asindirecte measure of NO release, was determined using Griess reactionand CXCL12 gene expression was measured by PCR. In vivo, ratsimmunized to develop EAE were treated with aminoguanidine, aninhibitor of iNOS activity, starting from day 9 post immunization,once per day until the disease peak was reached in control, untreatedgroup. At this point, CXCL12 gene expression was determined in SChomogenates and MBV.Results: We found negative correlation between gene expression ofiNOS and CXCL12 in SC of EAE rats. NO released from SNP orproduced by peritoneal cells in vitro significantly reduced CXCL12gene expression in astrocytes and MBV. In vivo inhibition of iNOS ledto upregulation of CXCL12 gene expression and reduction of EAEseverity in rats.Conclusions: These results imply that NO is important inhibitor ofCXCL12 expression within the CNS during the encephalitogenicautoimmune response. This adds to the list of harming effects of excessgeneration of NO in neuroinflammation
Amal Atia Mhfuod El- Muzghi, (9-2012)
staff photo

Dr. ADEL EMHIMMID MOHAMMED SAEIDAH

ADEL SAEIDAH is one of the staff members at the department of العام faculty of 13. He is working as a since 2017-10-22. He teaches several subjects in his major and has several puplications in the field of his interest.